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KMID : 1004820090100020069
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Journal of Biomedical Research
2009 Volume.10 No. 2 p.69 ~ p.78
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Influence of dietary iron on colonic aberrant crypt foci as a pre-neoplastic lesion induced by azoxymethane in mice
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Hue Jin-Joo
Kim Jun-Hyeong
Nam Sang-Yoon
Yun Young-Won
Lee Beom-Jun
Kim Jong-Soo
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Abstract
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A level of dietary iron may play a role in colon carcinogenesis. The effect of dietary iron oncolon carcinogenesis was investigated in male ICR mice. Five-week old mice were acclimated for one weekand fed on iron-normal diet (35 ppm Fe), iron-deficient diet (3 ppm), or iron-overloaded diet (350 ppm Fe) for8 weeks. Animals received three (0-2nd weeks after starting experiment) injections of azoxymethane (AOM; 10mg/kg b.w.) to induce colonic aberrant crypt foci (ACF). There were five experimental groups including normalcontrol without AOM, AOM+iron-normal diet (AOM+NFe), AOM+iron-deficient (AOM+LFe), AOM+ironoverloadeddiet (AOM+HFe) groups. The total numbers of ACF and aberrant crypt (AC) were measured inthe colonic mucosa after staining with methylene blue. The blood and serum were analyzed with a blood celldifferential counter and an automatic serum analyzer. The hepatic iron levels were significantly dependent onthe presence of iron in the diets. Iron-deficient diet significantly decreased the several hematological values.The values of glutamic oxaloacetic transaminase (GOT) and glutamic pyruvate transaminase (GPT) werealso significantly decreased in iron-overloaded or iron-deficient diet groups, compared with normal iron dietgroup. Dietary iron-deficiency decreased the numbers of ACF (64.9) and AC (79.8) per colon by 20.6 and21.8%, respectively, compared with AOM+NFe group (72.4 ACF/colon and 90.3 AC/colon). However, ironoverloadeddiet increased ACF (82.9) and AC (96.0) induction by AOM, compared with normal iron diet.These results suggest that dietary iron can affect the colon carcinogenesis in the animal model of mice.
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KEYWORD
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Aberrant crypt foci, Azoxymethane, Colon cancer, Iron, Mice
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